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FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar <t>density</t> <t>(α-SMA)</t> in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.
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FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar density (α-SMA) in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.

Journal: Journal of cellular and molecular medicine

Article Title: Kindlin-3 Promotes Angiogenesis via Notch Signalling and Is Crucial for Functional Recovery Postmyocardial Infarction.

doi: 10.1111/jcmm.70494

Figure Lengend Snippet: FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar density (α-SMA) in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.

Article Snippet: Name of antibody Manufacturer Identifier Concentration Kindlin- 3 Affinity Biosciences DF13558 1:1000 (WB), 1:200 (IHC) VEGF Proteintech 19003- 1- AP 1:1000 (WB) β1- integrin Bioss bs- 0486R 1:2000 (WB), 1:400 (IHC) GAPDH Proteintech 6004- 1- Ig 1:2000 (WB) Hif- 1α Affinity Biosciences BF8002 1:2000 (WB) eNOS Proteintech 27120- 1- AP 1:1000 (WB) p- eNOS Proteintech 28937- 1- AP 1:1000 (WB) CD31 Proteintech 11265- 1- AP 1:1000 (WB), 1:400 (IF) Cleaved Caspase 3 Cell Signaling Technology 9664 1:1000 (WB) Caspase 3 Cell Signaling Technology 9662 1:1000 (WB) α- Actinin Sigma A7811 1:400 (IF) α- SMA Proteintech 14395- 1- AP 1:400 (IF) vWF Proteintech 66682- 1- Ig 1:400 (IF) Notch1 Proteintech 20687- 1- AP 1:1000 (WB), 1:400 (IHC) Hes1 Affinity Biosciences DF7569 1:1000 (WB) HRP- conjugated a Goat Anti- Rabbit Servicebio GB233303 1:200 (IHC) Goat Anti- Rabbit IgG, Peroxidase Conjugated, H + L Biosharp BL003A 1:10000 (WB) Goat Anti- Mouse IgG, Peroxidase Conjugated, H + L Biosharp BL001A 1:10000 (WB) Alexa Fluor 488- conjugated AffiniPure Goat AntiRabbit IgG (H + L) Jackson 115- 585- 003 1:200 (IF) Alexa Fluor 594- conjugated AffiniPure Goat AntiMouse IgG (H + L) Jackson 115- 585- 003 1:200 (IF) Abbreviations: IF, immunofluorescence; IHC, immunohistochemistry; WB, Western blot. nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70494 by IN A SP - N E PA L , W iley O nline L ibrary on [21/03/2025].

Techniques: In Vivo, Staining, Immunofluorescence, Western Blot, Expressing